A Parallel Array Microfluidic Blood-brain Barrier Model for High-throughput Quantitation of Shear Stress Effects
نویسندگان
چکیده
In response to demand for innovative in vitro models for central nervous system disease and treatment we previously reported the microfluidic blood-brain barrier (μBBB). To further investigate shear stress, a key factor distinguishing the μBBB from traditional models, we substituted the top channel with independently monitored channel arrays to aid in high-throughput analysis. Optimal trans-endothelial electrical resistance (TEER) of 345Ωcm was achieved in channels exposed to shear stress in the range of 0.6-1.2dyn/cm, while decreased permeabilities of fluorescent tracers were seen at 1.17dyn/cm compared to 0.078dyn/cm, with an average decrease of 2ecm/s, indicating optimal flow conditions for subsequent μBBB studies.
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